PAX Blood RNA Kit(For PAXgene Blood RNA Tube)
目录号：91419
产品简介
PAX Blood Total RNA Kit（离心柱）用于配套PAX Blood RNA Kit使用。PAX Blood Total RNA Kit包含血液采集、稳定和运输的PAXgene Blood RNA Tubes（由BD提供，货号762165）, 以及RNA吸附柱技术的分离纯化组分。纯化可以使用离心机手工操作，也可以在QIAcube全自动核酸纯化仪上自动运行。该产品表现卓越，比同类产品提取的总RNA产量高一倍以上。
产品特点
适用于配套PAX Blood RNA Kit使用。
Kit Contents and Storage
|
Kit Contents |
Storage |
50 Preps (91419-50) |
|
Resuspension Buffer |
RT |
20 ml |
|
Binding Buffer |
RT |
50 ml |
|
Buffer RW1 |
RT |
40 ml |
|
Wash Buffer RW |
RT |
10 ml Add indicated ethanol before first use |
|
RNase-free H2O |
RT |
10 ml |
|
DNase Buffer |
–20 °C |
1.5 ml x 2 |
|
RNase free DNase I |
–20 °C |
250 μl |
|
Proteinase K (20mg / ml) |
–20 °C |
20 mg x 1 tube |
|
RNA Binding Columns |
RT |
50 |
注意：
蛋白酶K是一种冻干物。离心几秒钟，然后用1 ml蒸馏水，等分试样溶液。在–20 °C 下储存。 避免反复冻结和解冻。
所有试剂在指定温度下储存时，可稳定保存 9 个月。
Description
PAX Blood RNA Kit is designed for isolation of total RNA from blood samples stored in preservation reagents and Blood RNA Tubes. This procedure completely removes contaminants and enzyme inhibitors producing high-quality RNA. RNA purified using the PAX Blood RNA Kit is ready for applications such as RT-PCR.
The samples are removed from the preservation reagents. For blood samples stored in Blood RNA Tubes, the cells are collected by centrifugation. Samples are washed and lysed under an optimized buffer containing Proteinase K. The samples are centrifuged to remove cell debris and other particulates. After adjusting the binding conditions with ethanol, the samples are loaded on the RNA binding column. With a brief centrifugation or vacuum step, the samples pass through the column matrix which binds the RNA. Genomic DNA is removed with an on-the-column DNase I digestion treatment. After three wash steps, purified RNA is eluted with RNase-free water.